Application

Research CategorySignaling

Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)

Immunoprecipitation (Use Protein G): 2µg antibody/mg protein lysate.

Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.

Optimal working dilutions must be determined by end user.

Research Sub CategoryInsulin/Energy Signaling

Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Format: Purified

Specificity

Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.

Weakly reacts with Rabbit.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.